Ateneo

Laboratorio di Enzimologia e Biologia Molecolare Clinica

Attività di ricerca

Il gruppo coordinato dalla Prof.ssa Monica Emanuelli svolge la sua attività di ricerca presso il Laboratorio di Enzimologia e Biologia Molecolare Clinica, che ospita attrezzature e strumenti utili a studi nell’ambito della Biochimica, della Biologia Molecolare e della Biochimica Clinica.

Le principali attività del laboratorio sono relative a:

  • Caratterizzazione molecolare e cinetica di enzimi del metabolismo piridinico, in particolare dell’enzima Nicotinamide N-Metiltrasferasi (NNMT), coinvolto nei processi di biotrasformazione di farmaci e composti xenobiotici.

Peng Y, Sartini D, Pozzi V, Wilk D, Emanuelli M, Yee VC. Biochemistry, 2011

  • Analisi dei livelli di espressione dell’NNMT in differenti patologie tumorali e delle ricadute del silenziamento dell’enzima sul potenziale tumorigenico della cellula neoplastica.
  • Progettazione e sintesi di inibitori dell’nnMT con possibili applicazioni terapeutiche.

Gao Y, van Haren MJ, Buijs N, Innocenti P, Zhang Y, Sartini D, Campagna R, Emanuelli M, Parsons RB, Jespers W, Gutiérrez-de-Terán H, van Westen GJP, Martin NI. J Med Chem, 2021

  • Analisi dell’espressione dell’enzima Paraoxonasi-2 (PON2), coinvolto nei meccanismi di protezione cellulare dallo stress ossidativo, nelle cellule tumorali e del ruolo di tale attività catalitica nella resistenza ai farmaci ad azione antineoplastica.

Campagna R, Pozzi V, Giorgini S, Morichetti D, Goteri G, Sartini D, Serritelli EN, Emanuelli M. Hum Cell, 2023

  • Identificazione e validazione di biomarcatori enzimatici in fluidi biologici, per la diagnosi precoce e non invasiva del carcinoma della vescica.

Pozzi V, Di Ruscio G, Sartini D, Campagna R, Seta R, Fulvi P, Vici A, Milanese G, Brandoni G, Galosi AB, Montironi R, Cecati M, Emanuelli M. Int J Biol Markers, 2018

  • Studio dei marcatori prognostici delle lesioni preneoplastiche e neoplastiche del cavo orale.

Attrezzature

La strumentazione del Laboratorio è la seguente:

  • Biorad CFX96 Real-Time System C1000 Thermal Cycler
  • Bio-Rad ChemiDoc XRS+
  • High Performance Liquid Chromatography System (HPLC) Shimadzu
  • AB Applied Biosystems 2720 Thermal Cycler
  • Eppendorf Centrifuge 5418
  • Bio-Rad PowerPac Basic power supply per elettroforesi
  • Mini-sub cell GT Sistema per Western Blot Bio-Rad
  • Centrifuga Thermo Fisher Scientific Sorvall RC 6+
  • Eppendorf Ultra-Low temperature freezer CryoCube F440n.

Il personale del laboratorio può anche utilizzare le seguenti attrezzature:

  • Lettore Micropiastre Synergy HT, BioTEK
  • Ultracentrifuga Beckman Optima L-90K
  • Citofluorimetro Millipore Guava easyCyte
  • BioTek Lionheart FX Automated Microscope
  • Cappa a flusso laminare per colture cellulari FASTER srl BHA 48
  • Microscopio invertito Olympus CK2
  • Incubatore Thermo Scientific BB15 Heraeus,
  • Incubatore Thermo Scientific HERACell 150i a flusso controllato di ossigeno.
Biorad CFX96 Real-Time System C1000 Thermal Cycler
Bio-Rad ChemiDoc XRS+
High Performance Liquid Chromatography
System (HPLC) Shimadzu
AB Applied Biosystems 2720 Thermal cycler
Eppendorf Centrifuge 5418
Bio-Rad PowerPac Basic power supply per elettroforesi
Centrifuga Thermo Fisher Scientific Sorvall RC 6+
Eppendorf Ultra-low temperature freezer CryoCube F440n

Personale

Collaborazioni

Prof. Nathaniel I. Martin and Dr. Matthijs J. van Haren
Institute of Biology Leiden, Leiden University, Netherlands.

Prof. Richard B. Parsons
Institute of Pharmaceutical Science, King’s College London, London, UK.

Prof. Stefan Chlopicki
Jagiellonian Centre for Experimental Therapeutics, Krakòw, Poland.

Prof. Lorenzo Lo Muzio
Dipartimento di Medicina Clinica e Sperimentale, Università di Foggia, Foggia, Italia.

Prof. Giorgio Mori
Dipartimento di Medicina Clinica e Sperimentale, Università di Foggia, Foggia, Italia.

Prof.ssa Claudia Matteucci
Dipartimento di Medicina Sperimentale, Università Tor Vergata, Roma, Italia.

Pubblicazioni (2019 – 2024)

  1. Campagna R, Serritelli EN, Salvolini E, Schiavoni V, Cecati M, Sartini D, Pozzi V, Emanuelli M (2024). Contribution of the Paraoxonase-2 Enzyme to Cancer Cell Metabolism and Phenotypes. BIOMOLECULES, vol. 14, ISSN: 2218-273X.
  2. Pozzi V, Molinelli E, Campagna R, Serritelli EN, Cecati M, De Simoni E, Sartini D, Goteri G, Martin NI, van Haren MJ, Salvolini E, Simonetti O, Offidani A, Emanuelli M (2024). Knockdown of nicotinamide N-methyltransferase suppresses proliferation, migration and chemoresistance of Merkel cell carcinoma cells in vitro. HUMAN CELL, ISSN: 0914-7470.
  3. Serritelli EN, Sartini D, Campagna R, Pozzi V, Martin NI, van Haren MJ, Salvolini E, Cecati M, Rubini C, Emanuelli M (2024). Targeting nicotinamide N-methyltransferase decreased aggressiveness of osteosarcoma cells. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, ISSN: 1365-2362.
  4. Pozzi V, Campagna R, Sartini D, Emanuelli M (2023). Enzymes Dysregulation in Cancer: From Diagnosis to Therapeutical Approaches. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, ISSN: 1661-6596.
  5. Sartini, Davide, Molinelli, Elisa, Pozzi, Valentina, Campagna, Roberto, Salvolini, Eleonora, Rubini, Corrado, Goteri, Gaia, Simonetti, Oriana, Campanati, Anna, Offidani, Annamaria, Emanuelli, Monica (2023). Immunohistochemical expression of nicotinamide N-methyltransferase in lymph node metastases from cutaneous malignant melanoma. HUMAN CELL, vol. 36, p. 480-482, ISSN: 0914-7470, doi: 10.1007/s13577-022-00793-3.
  6. Campagna R, Pozzi V, Salvucci A, Togni L, Mascitti M, Sartini D, Salvolini E, Santarelli A, Lo Muzio L, Emanuelli M (2023). Paraoxonase-2 expression in oral squamous cell carcinoma. HUMAN CELL, vol. 36, p. 1211-1213, ISSN: 0914-7470.
  7. Campagna R, Pozzi V, Giorgini S, Morichetti D, Goteri G, Sartini D, Serritelli EN, Emanuelli M (2023). Paraoxonase-2 is upregulated in triple negative breast cancer and contributes to tumor progression and chemoresistance. HUMAN CELL, vol. 36, p. 1108-1119, ISSN: 0914-7470.
  8. Schiavoni V, Campagna R, Pozzi V, Cecati M, Milanese G, Sartini D, Salvolini E, Galosi AB, Emanuelli M (2023). Recent Advances in the Management of Clear Cell Renal Cell Carcinoma: Novel Biomarkers and Targeted Therapies. CANCERS, vol. 15, ISSN: 2072-6694.
  9. Campagna R, Belloni A, Pozzi V, Salvucci A, Notarstefano V, Togni L, Mascitti M, Sartini D, Giorgini E, Salvolini E, Santarelli A, Lo Muzio L, Emanuelli M (2023). Role Played by Paraoxonase-2 Enzyme in Cell Viability, Proliferation and Sensitivity to Chemotherapy of Oral Squamous Cell Carcinoma Cell Lines. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, 338, ISSN: 1422-0067, doi: 10.3390/ijms24010338.
  10. Memè L, Bambini F, Gallusi G, Sartini D, Pozzi V, Emanuelli M, Strappa EM, Mummolo S (2023). The Effect and the Potential Use of Magnetic–Dam Barrier in Guided Bone Regeneration: A Laboratory Study. APPLIED SCIENCES, vol. 13, ISSN: 2076-3417.
  11. Bacchetti T, Campagna R, Sartini D, Cecati M, Morresi C, Bellachioma L, Martinelli E, Rocchetti G, Lucini L, Ferretti G, Emanuelli M (2022). C. spinosa L. subsp. rupestris Phytochemical Profile and Effect on Oxidative Stress in Normal and Cancer Cells. MOLECULES, vol. 27, ISSN: 1420-3049.
  12. Zhurakivska K, Risteli M, Salo T, Sartini D, Salvucci A, Troiano G, Lo Muzio L, Emanuelli M. (2022). Effects of Fermented Wheat Germ Extract on Oral Cancer Cells: An In Vitro Study. NUTRITION AND CANCER, vol. 74, p. 2133-2141, ISSN: 0163-5581.
  13. Meme’, Lucia, Sartini, Davide, Pozzi, Valentina, Emanuelli, Monica, Strappa, Enrico M, Bittarello, Paolo, Bambini, Fabrizio, Gallusi, Gianni (2022). Epithelial Biological Response to Machined Titanium vs. PVD Zirconium-Coated Titanium: An In Vitro Study. MATERIALS, vol. 15, ISSN: 1996-1944.
  14. Pozzi V, Campagna R, Sartini D, Emanuelli M (2022). Nicotinamide N-Methyltransferase as Promising Tool for Management of Gastrointestinal Neoplasms. BIOMOLECULES, vol. 12, ISSN: 2218-273X.
  15. Cecati Monia, Vignini Arianna, Borroni Francesca, Pugnaloni Sofia, Alia Sonila, Sabbatinelli Jacopo, Nicolai Giulia, Taus Marina, Santarelli Andrea, Fabri Mara, Mazzanti Laura, Emanuelli Monica (2022). TAS1R3 and TAS2R38 Polymorphisms Affect Sweet Taste Perception: An Observational Study on Healthy and Obese Subjects. NUTRIENTS, ISSN: 2072-6643, doi: 10.3390/nu14091711.
  16. Emanuelli M, Sartini D, Molinelli E, Campagna R, Pozzi V, Salvolini E, Simonetti O, Campanati A, Offidani A (2022). The Double-Edged Sword of Oxidative Stress in Skin Damage and Melanoma: From Physiopathology to Therapeutical Approaches. ANTIOXIDANTS, vol. 11, ISSN: 2076-3921.
  17. Campagna R, Pozzi V, Sartini D, Salvolini E, Brisigotti V, Molinelli E, Campanati A, Offidani A, Emanuelli M (2021). Beyond Nicotinamide Metabolism: Potential Role of Nicotinamide N‐Methyltransferase as a Biomarker in Skin Cancers. CANCERS, vol. 13, ISSN: 2072-6694.
  18. Sartini D, Campagna R, Lucarini G, Pompei V, Salvolini E, Mattioli-Belmonte M, Molinelli E, Brisigotti V, Campanati A, Bacchetti T, Ferretti G, Offidani A, Emanuelli M (2021). Differential immunohistochemical expression of paraoxonase-2 in actinic keratosis and squamous cell carcinoma. HUMAN CELL, vol. 34, p. 1929-1931, ISSN: 0914-7470.
  19. Van Haren MJ, Gao Y, Buijs N, Campagna R, Sartini D, Emanuelli M, Mateuszuk L, Kij A, Chlopicki S, Escudé Martinez de Castilla P, Schiffelers R, Martin NI (2021). Esterase-Sensitive Prodrugs of a Potent Bisubstrate Inhibitor of Nicotinamide N-Methyltransferase (NNMT) Display Cellular Activity. BIOMOLECULES, vol. 11, ISSN: 2218-273X.
  20. Van Haren MJ, Zhang Y, Thijssen V, Buijs N, Gao Y, Mateuszuk L, Fedak FA, Kij A, Campagna R, Sartini D, Emanuelli M, Chlopicki S, Jongkees SAK, Martin NI (2021). Macrocyclic peptides as allosteric inhibitors of nicotinamide N-methyltransferase (NNMT). RSC CHEMICAL BIOLOGY, vol. 2, p. 1546-1555, ISSN: 2633-0679.
  21. Togni L, Mascitti M, Sartini D, Campagna R, Pozzi V, Salvolini E, Offidani A, Santarelli A, Emanuelli M (2021). Nicotinamide N-Methyltransferase in Head and Neck Tumors: A Comprehensive Review. BIOMOLECULES, vol. 11, ISSN: 2218-273X.
  22. Campagna R, Salvolini E, Pompei V, Pozzi V, Salvucci A, Molinelli E, Brisigotti V, Sartini D, Campanati A, Offidani A, Emanuelli M (2021). Nicotinamide N-methyltransferase gene silencing enhances chemosensitivity of melanoma cell lines. PIGMENT CELL & MELANOMA RESEARCH, vol. 34, p. 1039-1048, ISSN: 1755-148X, doi: 10.1111/pcmr.12993.
  23. Campagna R, Mateuszuk Ł, Wojnar-Lason K, Kaczara P, Tworzydło A, Kij A, Bujok R, Mlynarski J, Wang Y, Sartini D, Emanuelli M, Chlopicki S (2021). Nicotinamide N-methyltransferase in endothelium protects against oxidant stress-induced endothelial injury. BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR CELL RESEARCH, vol. 1868, ISSN: 1879-2596, doi: 10.1016/j.bbamcr.2021.119082.
  24. Bacchetti T, Salvolini E, Pompei V, Campagna R, Molinelli E, Brisigotti V, Togni L, Lucarini G, Sartini D, Campanati A, Mattioli-Belmonte M, Rubini C, Ferretti G, Offidani A, Emanuelli M (2021). Paraoxonase-2: A potential biomarker for skin cancer aggressiveness. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, vol. 51, ISSN: 1365-2362, doi: 10.1111/eci.13452.
  25. Gao Y, van Haren MJ, Buijs N, Innocenti P, Zhang Y, Sartini D, Campagna R, Emanuelli M, Parsons RB, Jespers W, Gutiérrez-de-Terán H, van Westen GJP, Martin NI (2021). Potent Inhibition of Nicotinamide N-Methyltransferase by Alkene-Linked Bisubstrate Mimics Bearing Electron Deficient Aromatics. JOURNAL OF MEDICINAL CHEMISTRY, vol. 64, p. 12938-12963, ISSN: 1520-4804.
  26. Campanati A, Martina E, Diotallevi F, Radi G, Marani A, Sartini D, Emanuelli M, Kontochristopoulos G, Rigopoulos D, Gregoriou S, Offidani A (2021). Saliva proteomics as fluid signature of inflammatory and immune mediated skin diseases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 22, ISSN: 1422-0067.
  27. Campagna R, Pozzi V, Spinelli G, Sartini D, Milanese G, Galosi AB, Emanuelli M (2021). The Utility of Nicotinamide N-Methyltransferase as a Potential Biomarker to Predict the Oncological Outcomes for Urological Cancers: An Update. BIOMOLECULES, vol. 11, ISSN: 2218-273X.
  28. Togni L., Mascitti M., Vignigni A., Alia S., Sartini D., Barlattani A., Emanuelli M., Santarelli A. (2021). Treatment‐related dysgeusia in oral and oropharyngeal cancer: A comprehensive review. NUTRIENTS, vol. 13, ISSN: 2072-6643, doi: 10.3390/nu13103325.
  29. de Maria S, Santoro A, Fuggetta MP, Rocchetti R, Cottarelli A, Lanzilli G, Stiuso P, Angelico G, Spadola S, Franco Zannoni G, Rubini C, Emanuelli M, Carmela Pedicillo M, Pannone G, Lo Muzio L (2020). A possible interplay between HR-HPV and stemness in tumor development: an in vivo investigation of CD133 as a putative marker of cancer stem cell in HPV18-infected KB cell line. APMIS. ACTA PATHOLOGICA, MICROBIOLOGICA ET IMMUNOLOGICA SCANDINAVICA, vol. 128, p. 637-646, ISSN: 0903-4641, doi: 10.1111/apm.13078.
  30. Fumarola S, Cecati M, Sartini D, Ferretti G, Milanese G, Galosi AB, Pozzi V, Campagna R, Morresi C, Emanuelli M, Bacchetti T. (2020). Bladder Cancer Chemosensitivity is Affected by Paraoxonase-2 Expression. ANTIOXIDANTS, vol. 9, ISSN: 2076-3921, doi: 10.3390/antiox9020175.
  31. Pozzi V, Salvolini E, Lucarini G, Salvucci A, Campagna R, Rubini C, Sartini D, Emanuelli M (2020). Cancer stem cell enrichment is associated with enhancement of nicotinamide N-methyltransferase expression. IUBMB LIFE, vol. 72, p. 1415-1425, ISSN: 1521-6543, doi: 10.1002/iub.2265.
  32. Mascitti M, Sartini D, Togni L, Pozzi V, Rubini C, Santarelli A, Emanuelli M (2020). Differential expression of nicotinamide N-methyltransferase in primary and recurrent ameloblastomas and odontogenic keratocysts. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, vol. 50, ISSN: 0014-2972, doi: 10.1111/eci.13220.
  33. Sartini D, Pompei V, Lucarini G, Rubini C, Molinelli E, Brisigotti V, Salvolini E, Campanati A, Offidani A, Emanuelli M (2020). Differential expression of nicotinamide n-methyltransferase in cutaneous keratoacanthoma and squamous cell carcinoma: an immunohistochemical study. JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, vol. 34, p. 121-123, ISSN: 1468-3083, doi: 10.1111/jdv.16048.
  34. Campagna R, Bacchetti T, Salvolini E, Pozzi V, Molinelli E, Brisigotti V, Sartini D, Campanati A, Ferretti G, Offidani A, Emanuelli M. (2020). Paraoxonase-2 Silencing Enhances Sensitivity of A375 Melanoma Cells to Treatment with Cisplatin. ANTIOXIDANTS, vol. 9, p. 1-13, ISSN: 2076-3921, doi: 10.3390/antiox9121238.
  35. Mascitti M, Santarelli A, Sartini D, Rubini C, Colella G, Salvolini E, Ganzetti G, Offidani A, Emanuelli M (2019). Analysis of nicotinamide N-methyltransferase in oral malignant melanoma and potential prognostic significance. MELANOMA RESEARCH, vol. 29, p. 151-156, ISSN: 0960-8931, doi: 10.1097/CMR.0000000000000548.
  36. Gao Y, van Haren MJ, Moret EE, Rood JJM, Sartini D, SALVUCCI, ALESSIA, Emanuelli M, Craveur P, Babault N, Jin J, Martin NI (2019). Bisubstrate Inhibitors of Nicotinamide N-Methyltransferase (NNMT) with Enhanced Activity. JOURNAL OF MEDICINAL CHEMISTRY, vol. 62, p. 6597-6614, ISSN: 0022-2623, doi: 10.1021/acs.jmedchem.9b00413.
  37. Morresi C, Cianfruglia L, Sartini D, Cecati M, Fumarola S, Emanuelli M, Armeni T, Ferretti G, Bacchetti T (2019). Effect of High Glucose-Induced Oxidative Stress on Paraoxonase 2 Expression and Activity in Caco-2 Cells. CELLS, vol. 8, ISSN: 2073-4409, doi: 10.3390/cells8121616.
  38. Pompei V, Salvolini E, Rubini C, Lucarini G, Molinelli E, Brisigotti V, Pozzi V, Sartini D, Campanati A, Offidani A, Emanuelli M (2019). Nicotinamide N-methyltransferase in nonmelanoma skin cancers. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, vol. 49, ISSN: 0014-2972, doi: 10.1111/eci.13175.
  39. Salvolini E, Vignini A, Sabbatinelli J, Lucarini G, Pompei V, Sartini D, Cester AM, Ciavattini A, Mazzanti L, Emanuelli (2019). Nitric oxide synthase and VEGF expression in full-term placentas of obese women. HISTOCHEMISTRY AND CELL BIOLOGY, vol. 152, p. 415-422, ISSN: 1432-119X, doi: 10.1007/s00418-019-01819-y.
  40. Seta R, Mascitti M, Campagna R, Sartini D, Fumarola S, Santarelli A, Giuliani M, Cecati M, Muzio LL, Emanuelli M (2019). Overexpression of nicotinamide N-methyltransferase in HSC-2 OSCC cell line: effect on apoptosis and cell proliferation. CLINICAL ORAL INVESTIGATIONS, vol. 23, p. 829-838, ISSN: 1432-6981, doi: 10.1007/s00784-018-2497-8.